Abstract and keywords
Abstract (English):
It is a matter of common knowledge that C60 fullerenes and their derivatives have antioxidant properties due to their chemical structure. Studies show that these substances also possess antiviral and anticancer activity, moreover, they are under studies as potential nanocarriers for drug delivery across biological barriers. But the effect of such derivatives on human cells is still controversially discussed and needs further investigation. Here we assessed the time-dependent effect of a water soluble fullerene derivative that contains five residues of 3-phenylpropionic acid and a chlorine substituent attached to the carbon cage (F-828) on human embryonic lung fibroblasts (HELFs). Investigated substance increases proliferation of HELFs in concentration range 4 nM - 0.1 µM. All concentrations of F-828 cause DNA damage and give rise to reparation process. In concentrations exceeding 20 µM F-828 causes necrotic cell death, blocks apoptosis, and is therefore cytotoxic.

fullerene derivatives, necrosis, HELFs
Publication text (PDF): Read Download

1. Yang X., Ebrahimi A., Li J., Cui Q. Fullerene-biomolecule conjugates and their biomedicinal applications, Int J Nanomedicine, 2014, vol. 9, pp. 77-92.

2. Grebowski J., Kazmierska P., Krokosz A. Fullerenols as a new therapeutic approach in nanomedicine, Biomed Res Int [Online], 2013, r. 751913.

3. Nakamura S., Mashino T. Water-soluble fullerene derivatives for drug discovery, J Nippon Med Sch., 2012, vol. 79, pp. 248-254.

4. Bullard-Dillard R., Creek K.E., Scrivens W.A., Tour J.M., Tissue sites of uptake of 14C labelled C60. Bioorg. Chem., 1996, vol. 24, no. 4, pp. 376-385.

5. Rouse J.G., Yang J., Barron A.R., Monteiro-Riviere N.A. Fullerene-based amino acid nanoparticle interactions with human epidermal keratinocytes. Toxicol. In Vitro, 2006, vol. 20, no. 8, pp. 1313-1320.

6. Sayes C.M., Marchione, A.A., Reed K.L., Warheit D.B. Comparative pulmonary toxicity assessments of C60 water suspensions in rats: few differences in fullerene toxicity in vivo in contrast to in vitro profiles. Nano Lett., 2007, vol. 7, no. 8, pp. 2399-2406.

7. Vistica D.T., Skehan P., Scudiero D., Monks A., Pittman A., Boyd M.R. Tetrazolium-based assays for cellular viability: a critical examination of selected parameters affecting formazan production. Cancer Res., 1991, vol. 51, pp. 2515-2520.

8. Holder A.L., Goth-Goldstein R., Lucas D., Koshland C.P. Particle-induced artifacts in the MTT and LDH viability assays. Chem Res Toxicol, 2012, vol. 17, pp. 1885-1892.

9. Monteiro-Riviere N.A., Inman A.O., Zhang L.W. Limitations and relative utility of screening assays to assess engineered nanoparticle toxicity in a human cell line. Toxicol Appl Pharmacol, 2009, vol. 234, pp. 222-235.

10. Moore J.O., Palep S.R., Saladi R.N., Gao D., Wang Y., Phelps R.G., Lebwohl M.G., Wei H. Effects of ultraviolet B exposure on the expression of proliferating cell nuclear antigen in murine skin. Photochem Photobiol, 2004, vol. 80, pp. 587-595.

11. Shivji K.K., Kenny M.K., Wood R.D. Proliferating cell nuclear antigen is required for DNA excision repair. Cell, 1992, vol. 69, pp. 367-374.

12. Löbrich M., Shibata A., Beucher A., Fisher A., Ensminger M., Goodarzi A.A., Barton O., Jeggo P.A. GammaH2AX foci analysis for monitoring DNA doublestrand break repair: strengths, limitations and optimization. Cell Cycle, 2010, vol. 9, pp. 662-669.

Login or Create
* Forgot password?